MASH-FRET is a Matlab-based software package for the analysis of single-molecule FRET videos and trajectories. The framework encompasses the entire workflow from localizing single molecule on videos to characterizing molecule dynamics.
The individual modules of MASH-FRET are documented here. Step-by-step tutorials guide you through the entire workflow from picking single-molecule all the way to determining state populations.
MASH-FRET is available on GitHub. The software is updated annually, tested regularly, and we are working to improve it continuously. Feedback and bugs can be reported via GitHub as well.